Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis of HepG2 cell lysate (35 ug/lane) with NEUROD1 polyclonal antibody (Cat # PAB3857).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEUROD1 polyclonal antibody (Cat # PAB3857) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma

Immunofluorescence
Application

Immunofluorescence

ES cells were transiently transfected with flag-tagged mouse NEUROD1.
Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NEUROD1 polyclonal antibody (Cat # PAB3857) at 1:100. NEUROD1 polyclonal antibody (Cat # PAB3857) showed strong and clear staining with similar pattern to the flag staining.
(Supplied by Sally Lowell, Edinburgh University)

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of NEUROD1.

    Immunogen

    A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NEUROD1.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Purification

    Protein G purification

    Recommend Usage

    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot analysis of HepG2 cell lysate (35 ug/lane) with NEUROD1 polyclonal antibody (Cat # PAB3857).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEUROD1 polyclonal antibody (Cat # PAB3857) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma

    Immunofluorescence

    ES cells were transiently transfected with flag-tagged mouse NEUROD1.
    Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NEUROD1 polyclonal antibody (Cat # PAB3857) at 1:100. NEUROD1 polyclonal antibody (Cat # PAB3857) showed strong and clear staining with similar pattern to the flag staining.
    (Supplied by Sally Lowell, Edinburgh University)
  • Gene Info — NEUROD1

    Entrez GeneID

    4760

    Protein Accession#

    NP_002491;Q13562

    Gene Name

    NEUROD1

    Gene Alias

    BETA2, BHF-1, NEUROD, bHLHa3

    Gene Description

    neurogenic differentiation 1

    Omim ID

    125853 601724

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq

    Other Designations

    basic helix-loop-helix transcription factor|beta-cell E-box transactivator 2|neurogenic differentiation factor 1|neurogenic helix-loop-helix protein NEUROD

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