Disease:

Epithelioid carcinoma of the pancreas

Origin:

established in 1972 from the ductal cells of a tumor located in the head of the pancreas of a 56-year-old man; the cells are described to be tumorigenic in nude-athymic mouse.

Species:

Homo sapiens

Tissue:

Pancreas, duct

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%. 2n = 46, hypertriploid

Patient:

Male, Caucasian, 56 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:2 to 1:4; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO