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> PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)

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Referencia 31033

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PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.

PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

Product Attributes

Viability dye

PMA, PMAxx™
Storage Conditions

Store at -10 to -35 °C
Assay type/options

Live/dead discrimination, Viability PCR
Detection method/readout

PCR/qPCR

Technology

Microbiology Products
Viability PCR

The Full Spectrum Blog

Blog: 12 years later: Viability PCR using PMA and PMAxx™ dyes
Blog: Switching from agar plates to v-PCR for bacteria viability testing – What you need to know

PMA is stable after dilution to 0.2 mM in water as long as it is protected from light and can be stored in the same way as the 20 mM stock solution.


View more FAQs
What is the procedure for decontaminating the PMA-Lite™ 2.0 LED Photolysis Device?

Note: Do not remove the cover or introduce liquids to the interior of the PMA-Lite.

  1. Thoroughly wipe all exposed PMA-Lite surfaces and the inner rims of the tube holes with 10% bleach in water (household bleach diluted at a ratio of 1 part bleach to 9 parts water).
  2. Let the bleach sit on the unit for 10 minutes.
  3. Thoroughly wipe the surfaces with dH2O.
  4. Wipe the surfaces with 70% ethanol and allow to air dry.


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What’s the difference between EMA, PMA, and PMAxx™?

Viability PCR originally used EMA (ethidium monoazide) to inactivate dead cell DNA. Biotium developed PMA (propidium monoazide) in collaboration with investigators at Montana State University (Nocker et al. 2006). PMA is more selective for dead cells than EMA, and became widely used for selective detection of viable microbes and viruses. PMAxx™ is Biotium’s newest viability PCR dye, designed to be more effective than PMA at eliminating PCR amplification of dead cell DNA. Therefore it provides the best discrimination between live and dead bacteria.

Learn more about PMA and PMAxx™ for viability PCR.


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Protocols
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Supporting Documents

Product Description

This kit is designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the invA gene.

Features

  • Selectively detect viable Salmonella enterica using qPCR
  • Contains primers to amplify the invA gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
  • Sufficient reagents for 200 PCR reactions

Kit Components

  • PMA dye or PMAxx™ dye
  • PMA Enhancer for Gram Negative Bacteria
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • invA primer mix, 5 uM

About this kit

Salmonella enterica is a gram-negative bacteria that causes the food-borne illness salmonellosis. PMA-based viability PCR for S. enterica has been reported using the primers provided in the kit. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria Strain Gene name Kit with PMA (catalog #) Kit with PMAxx™ (catalog #)
Salmonella enterica invA 31033 31033-X
Mycobacterium tuberculosis groEL2 31034 N/A
Staphylococcus aureus nuc 31035 N/A
MRSA mecA 31036 N/A
E. coli O157:H7 Z3276 31037 31037-X
E. coli uidA 31050 31050-X
Listeria monocytogenes hly 31051 31051-X
Legionella pneumophila mip 31053 N/A

Don’t see your favorite strain? Let us know at info@quimigen.pt.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.

 
A multi-functional blue LED light box that can be used for photolysis of PMAxx™ or PMA in viability PCR; or to develop the visible DNA gel stain DNAzure®.
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PMA Enhancer for Gram Negative Bacteria, 5X Solution

This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.
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PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.
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PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.
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PMA-Lite™ 2.0 LED Photolysis Device

An optimized photoactivation device designed for photolysis of PMAxx™- and PMA- treated samples in viability PCR.
Detailed View

PMAxx™ Dye, 20 mM in H2O

Viability PCR dye, a new and improved version of the popular viability dye propidium monoazide (PMA).
Detailed View
31033

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