Mixture of at least 10 proteases: five serine type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases and one zinc carboxypeptidase. Digestion with the product has been useful when extensive or complete degradation of protein is required. Pronase E is used in tissue dissociation of various tissues, e.g. to isolate living chondrocytes.
Additional applications are the structural analysis of proteins (1, 2), preparation of bacteriophage lambda DNA (3), pretreatment of tissue sections to enhance the intensity of immunostaining and removal of protein in DNA/RNA isolations.

Activity: min. 5.0 DMC U/mg Unit definition: 1 DMC-U (1U) is that amount of enzymatic activity which catalyzes the cleavage of 1 µ-equivalent peptide bond from dimethyl casein per minute at 25 °C, pH 7.5, expressed in terms of the appearance of new terminal amino groups (4).
Activity in other units: ca. 1 000 000 PU-units/g (casein substrate; 40 °C, pH 7.4 (5), ca. 20 000 PUK-units/g (casein substrate, 40 °C, pH 7.5).
Pronase = registered trademark of Calbiochem-Novabiochem Corp.


References:

1. Jehanli, A. & Hough, D. (1985) Mol. Immunol. 22, 557-66
2. Tsugita, A. & Akabori, S. (1959) J. Biochem. (Tokyo) 46, 695-704
3. Maniatis, T. et al. (1982) Molecular Cloning - a Laboratory Manual. Cold Spring, Harbor Laboratory, p. 85
4. Lin, Y. et al. (1969) J. Biol. Chem. 244, 789-93
5. Nomoto, M. & Narashi, Y. (1959) J. Biochem. (Tokyo) 46, 653-67

DANGER

Hazard Statements	H315 - H319 - H334 - H335
Precautions	P280 - P285
Reaction	P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311

EG-Index: 647-014-00-9 • EINECS: 232-909-5 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C