Rat ANTI Human CD3 Antibody
Referencia OASA11552
embalaje : 25ug
Marca : Aviva Systems Biology
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Rat anti-Human CD3 Antibody (OASA11552)
| Datasheets/Manuals | Printable datasheet for Rat anti-Human CD3 Antibody (OASA11552) |
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| Predicted Species Reactivity | Human |
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| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | CD3-12 |
| Isotype | IgG1 |
| Host | Rat |
| Application | FC|IF|IHC-Fr|IHC-P |
| Additional Information | Histology Positive Control Tissue: Tonsil Carrier Free: Yes |
| Reconstitution and Storage | -20°C |
| Immunogen | Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC ) |
| Purification | Purified IgG prepared from tissue culture supernatant |
| Predicted Homology Based on Immunogen Sequence | Bovine, dog, horse, Rhesus monkey, pig, chicken, mouse, duck, koala, Harbour porpoise, alpaca, Cynomolgus monkey, spotted hyena, sea lion, cat, Amazon parrot, raccoon, great horned-owl, bullfrog, xenopus, rabbit. Based on sequence similarity, is expected to react with: mammals, birds, amphibia. Antibody reactivity and working conditions may vary between species. Antibody reactivity and working conditions may vary between species. |
| Concentration | 1 mg/ml |
| Specificity | Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3-epilson chain recognizes human CD3-epsilon. As this product has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. |
| Application Info | FC: 1/50-1/100. Membrane permeabilization is required for this application IHC-F: 1/100 IHC-P: 1/100. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer (pH 9.0) is recommended for this purpose. |
| Publications | Lack of Major Histocompatibility Complex Class I Upregulation and Restrictive Infection by JC Virus Hamper Detection of Neurons by T Lymphocytes in the Central Nervous System. J Neuropathol Exp Neurol. 74, 791-803 (2015). 2611519224128680 |
| Description |
| Reference | 1. Jones, M. et al. (1993). Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J. Immunol. 150 (12): 5429-5435. 2. Alterio, M. et al. (1998). Control of cytomeglavirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rest primarily on recipient- derived CD8 T cells. J. Virol. 72 (10): 7733 - 7744 3. Burundi, E. et al. (2002). Regulation of indoeamine 2, 3-diooxygenase expression in Simian Immunodefiency Virus-infected monkey brains. J. Virol. 76 (3): 12233 - 12241. |
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| Alias Symbols | CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), IMD18, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE. |
| NCBI Gene Id | 916 |
| Protein Name | T-cell surface glycoprotein CD3 epsilon chain |
| Description of Target | CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilon-gamma, epsilon-delta, zeta-zeta). The CD3 complex serves as a T-cell co-receptor that associates non-covalently with the T-cell receptor (TCR). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as a T-cell marker. |
| Uniprot ID | P07766 |
| Protein Accession # | NP_000724.1 |
