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> Rat CTSL (Cathepsin L) CLIA Kit

Rat CTSL (Cathepsin L) CLIA Kit

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Referencia E-CL-R0124-96T

embalaje : 96T

Marca : Elabscience

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Product Summary
Sensitivity 75 pg/mL
Detection Range 125-8000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Rat
Specificity This kit recognizes Rat CTSL in samples.No significant cross-reactivity or interference between Rat CTSL and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Chemiluminescence
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 6 months
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTSL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTSL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTSL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CTSL. You can calculate the concentration of Rat CTSL in the samples by comparing the RLU value of the samples to the standard curve.
Uniport ID P07154
Research Area Cancer , Neuroscience , Signal Transduction
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