Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit
Referencia KTE100502-48
embalaje : 48T
Marca : Abbkine
Specification
Product name | Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit |
Reactivity | Rat |
Applications | ELISA |
Applications notes | This Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit employs a two-site sandwich ELISA to quantitate PIIINP in samples. An antibody specific for PIIINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIIINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIIINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIIINP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method | Colorimetric |
SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
Assay type | Sandwich ELISA (quantitative) |
Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Alternative | PIIINP |
Product Properties
Kit components | minutes before opening. minutes. - 8 class="pro-form-title">Additional Information
Image & description |