RBM10 (NM_005676) Human Tagged ORF Clone
CAT#: RC200150
RBM10 (Myc-DDK-tagged)-Human RNA binding motif protein 10 (RBM10), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | RBM10 |
| Synonyms | DXS8237E; GPATC9; GPATCH9; S1-1; TARPS; ZRANB5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RC200150 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTATGAAAGACGTGGTGGTCGTGGTGACAGGACTGGCCGCTATGGAGCCACTGACCGCTCGCAGG ATGATGGTGGGGAGAACCGCAGCCGAGACCACGACTACCGGGACATGGACTACCGTTCATATCCTCGCGA GTATGGCAGCCAGGAGGGCAAGCATGACTATGACGACTCATCTGAGGAGCAGAGTGCGGAGGATTCCTAC GAGGCCTCCCCGGGCTCCGAGACTCAGCGTAGGCGGCGGCGGCGGCACAGGCACAGCCCCACCGGCCCGC CAGGCTTCCCCCGAGACGGCGACTATCGGGACCAGGACTATCGGACCGAGCAAGGGGAGGAGGAGGAGGA GGAGGAGGATGAGGAGGAGGAGGAGAAGGCCAGTAACATCGTCATGCTGAGGATGCTGCCACAGGCAGCC ACTGAGGATGACATCCGTGGCCAGCTGCAGTCGCACGGCGTGCAAGCACGGGAGGTTCGGCTGATGCGGA ACAAATCTTCAGGTCAGAGCCGGGGCTTCGCCTTCGTCGAGTTTAGTCACTTGCAGGACGCTACACGATG GATGGAAGCCAATCAGCACTCCCTCAACATCCTGGGCCAGAAGGTGTCGATGCACTACAGTGACCCCAAG CCCAAGATCAATGAGGACTGGCTGTGCAATAAGTGTGGCGTCCAGAACTTCAAACGCCGAGAGAAGTGCT TCAAATGTGGCGTGCCCAAGTCAGAGGCAGAGCAGAAGCTGCCCCTCGGCACGAGGCTGGATCAGCAGAC ACTGCCACTGGGTGGCCGGGAGCTGAGCCAGGGCCTGCTTCCCCTGCCGCAGCCCTACCAGGCCCAGGGA GTCCTGGCCTCCCAAGCCCTGTCACAGGGCTCGGAGCCAAGCTCAGAGAACGCCAATGACACCATCATTT TGCGCAACCTGAACCCACACAGCACCATGGATTCCATCCTGGGGGCCCTGGCACCCTACGCGGTGCTGTC CTCCTCCAACGTGCGCGTCATAAAGGACAAGCAGACCCAACTGAACCGCGGCTTTGCCTTCATCCAGCTC TCCACCATCGTGGAGGCAGCCCAGCTGCTGCAGATCCTGCAGGCCCTGCACCCACCACTCACTATCGACG GCAAGACCATCAATGTTGAGTTTGCCAAGGGTTCTAAGAGGGACATGGCCTCCAATGAAGGCAGTCGCAT CAGTGCTGCCTCTGTGGCCAGCACTGCCATTGCTGCGGCCCAGTGGGCCATCTCACAGGCCTCCCAAGGT GGGGAGGGTACCTGGGCCACCTCCGAGGAGCCGCCGGTCGACTACAGCTACTACCAACAGGATGAGGGCT ATGGCAACAGCCAGGGCACAGAGTCTTCCCTCTATGCCCATGGCTACCTCAAGGGCACCAAGGGCCCTGG CATCACTGGAACCAAAGGGGATCCCACTGGAGCAGGTCCCGAGGCCTCCCTAGAGCCTGGGGCCGACTCT GTGTCGATGCAGGCTTTCTCTCGCGCCCAGCCTGGTGCTGCTCCTGGCATCTACCAACAATCAGCCGAGG CGAGCAGTAGCCAGGGCACTGCTGCCAACAGCCAGTCGTATACCATCATGTCACCCGCTGTGCTCAAATC TGAGCTCCAGAGCCCTACCCATCCTAGTTCTGCTCTCCCACCGGCTACCAGCCCCACTGCCCAGGAATCC TACAGCCAGTACCCTGTTCCCGACGTCTCTACCTACCAGTACGATGAGACCTCCGGCTACTACTATGACC CCCAGACCGGCCTCTACTATGACCCCAACTCCCAGTATTACTACAATGCTCAGAGCCAGCAGTACCTGTA CTGGGATGGGGAGAGGCGGACCTATGTTCCCGCCCTGGAGCAGTCGGCCGACGGACATAAGGAGACAGGG GCACCCTCGAAGGAGGGCAAAGAGAAGAAGGAGAAGCACAAGACCAAGACAGCTCAACAGATTGCCAAGG ACATGGAACGCTGGGCCCGCAGTCTCAACAAACAAAAAGAAAACTTCAAAAATAGCTTCCAGCCTATCAG CTCCCTGCGAGATGACGAGAGGCGGGAGTCAGCCACTGCAGATGCTGGCTATGCCATCCTCGAGAAGAAG GGAGCACTAGCCGAGAGACAGCACACCAGCATGGATCTCCCGAAATTGGCCAGTGACGACCGCCCAAGCC CTCCGCGAGGACTGGTGGCAGCCTACAGCGGGGAGAGTGACAGTGAGGAGGAGCAGGAGCGTGGGGGCCC TGAGCGGGAGGAGAAGCTCACCGACTGGCAGAAGCTGGCCTGTCTGCTCTGCCGACGCCAGTTCCCCAGC AAAGAGGCGCTCATCCGGCACCAGCAGCTCTCAGGGCTCCACAAGCAAAACCTTGAGATTCACCGGCGAG CCCACTTGTCAGAAAACGAGCTAGAAGCACTAGAGAAGAATGACATGGAGCAAATGAAGTACCGGGACCG TGCAGCTGAACGCAGAGAAAAGTATGGCATCCCCGAGCCGCCAGAGCCCAAGAGGAGGAAGTACGGCGGC ATATCCACAGCCTCTGTAGACTTCGAGCAGCCTACTCGGGACGGGCTGGGCAGTGACAACATTGGCAGTC GGATGCTGCAGGCCATGGGCTGGAAAGAGGGCAGCGGCCTGGGCCGCAAGAAGCAGGGCATTGTAACGCC TATCGAGGCCCAAACACGGGTGCGGGGCTCCGGCCTGGGTGCACGGGGCAGCTCCTACGGGGTCACCTCA ACCGAGTCCTACAAGGAGACACTGCACAAGACAATGGTGACCCGCTTCAACGAGGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200150 protein sequence Red=Cloning site Green=Tags(s) MEYERRGGRGDRTGRYGATDRSQDDGGENRSRDHDYRDMDYRSYPREYGSQEGKHDYDDSSEEQSAEDSY EASPGSETQRRRRRRHRHSPTGPPGFPRDGDYRDQDYRTEQGEEEEEEEDEEEEEKASNIVMLRMLPQAA TEDDIRGQLQSHGVQAREVRLMRNKSSGQSRGFAFVEFSHLQDATRWMEANQHSLNILGQKVSMHYSDPK PKINEDWLCNKCGVQNFKRREKCFKCGVPKSEAEQKLPLGTRLDQQTLPLGGRELSQGLLPLPQPYQAQG VLASQALSQGSEPSSENANDTIILRNLNPHSTMDSILGALAPYAVLSSSNVRVIKDKQTQLNRGFAFIQL STIVEAAQLLQILQALHPPLTIDGKTINVEFAKGSKRDMASNEGSRISAASVASTAIAAAQWAISQASQG GEGTWATSEEPPVDYSYYQQDEGYGNSQGTESSLYAHGYLKGTKGPGITGTKGDPTGAGPEASLEPGADS VSMQAFSRAQPGAAPGIYQQSAEASSSQGTAANSQSYTIMSPAVLKSELQSPTHPSSALPPATSPTAQES YSQYPVPDVSTYQYDETSGYYYDPQTGLYYDPNSQYYYNAQSQQYLYWDGERRTYVPALEQSADGHKETG APSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKK GALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPS KEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGG ISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTS TESYKETLHKTMVTRFNEAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_005676 |
| ORF Size | 2790 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005676.5 |
| RefSeq Size | 3412 bp |
| RefSeq ORF | 2793 bp |
| Locus ID | 8241 |
| UniProt ID | P98175 |
| Cytogenetics | Xp11.3 |
| Domains | G-patch, RRM, zf-RanBP, zf-C2H2 |
| Protein Families | Druggable Genome |
| MW | 103.5 kDa |
| Gene Summary | This gene encodes a nuclear protein that belongs to a family proteins that contain an RNA-binding motif. The encoded protein associates with hnRNP proteins and may be involved in regulating alternative splicing. Defects in this gene are the cause of the X-linked recessive disorder, TARP syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC200150L1 | Lenti ORF clone of Human RNA binding motif protein 10 (RBM10), transcript variant 1, Myc-DDK-tagged | 1 151,00 EUR | |
| RC200150L2 | Lenti ORF clone of Human RNA binding motif protein 10 (RBM10), transcript variant 1, mGFP tagged | 1 151,00 EUR | |
| RC200150L3 | Lenti ORF clone of Human RNA binding motif protein 10 (RBM10), transcript variant 1, Myc-DDK-tagged | 1 151,00 EUR | |
| RC200150L4 | Lenti ORF clone of Human RNA binding motif protein 10 (RBM10), transcript variant 1, mGFP tagged | 1 151,00 EUR | |
| RG200150 | RBM10 (tGFP-tagged) - Human RNA binding motif protein 10 (RBM10), transcript variant 1 | 1 051,00 EUR | |
| SC109926 | RBM10 (untagged)-Human RNA binding motif protein 10 (RBM10), transcript variant 1 | 938,00 EUR |