RBM22 (NM_018047) Human Tagged ORF Clone
Referencia RC201492
embalaje : 10ug
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| Product Data | |
| Target Symbol | RBM22 |
|---|---|
| Synonyms | Cwc2; fSAP47; ZC3H16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC201492 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCTCTCTGGGTTCCAACACCTACAACAGGCAGAACTGGGAGGATGCGGACTTCCCCATTCTGT GCCAGACATGTCTTGGAGAAAACCCATATATCCGAATGACCAAAGAAAAGTATGGGAAGGAATGCAAAAT CTGTGCCAGGCCATTCACAGTGTTTCGCTGGTGCCCTGGAGTCCGCATGCGTTTCAAGAAGACTGAAGTG TGCCAAACCTGCAGTAAATTGAAGAATGTCTGTCAGACCTGCCTCTTAGACCTAGAGTATGGCCTGCCCA TCCAGGTTCGTGACGCAGGATTGTCTTTTAAAGATGACATGCCAAAGTCAGATGTCAACAAAGAGTACTA TACACAGAATATGGAGAGAGAGATTTCTAACTCTGATGGAACACGGCCAGTTGGCATGCTGGGGAAAGCC ACATCTACCAGTGACATGCTGCTCAAACTGGCCCGGACCACACCCTACTACAAAAGGAATCGACCCCACA TTTGCTCCTTCTGGGTGAAAGGAGAGTGTAAGAGAGGAGAGGAATGTCCATACAGACATGAGAAGCCTAC AGATCCAGATGACCCCCTTGCTGATCAGAATATTAAAGACCGTTATTACGGAATCAATGATCCTGTAGCT GACAAGCTTCTAAAGCGGGCTTCAACAATGCCTCGGCTGGACCCACCAGAGGATAAAACTATCACCACAC TATATGTTGGTGGTCTAGGTGATACCATTACTGAGACAGATTTAAGAAATCATTTCTACCAGTTCGGAGA GATCCGGACGATCACTGTTGTGCAGAGACAGCAGTGTGCTTTCATCCAGTTTGCCACACGGCAGGCTGCA GAAGTGGCTGCTGAGAAGTCCTTTAATAAGTTGATTGTAAATGGCCGCAGACTGAATGTGAAATGGGGAA GATCCCAGGCAGCCAGAGGAAAAGAAAAAGAGAAAGATGGAACTACAGACTCTGGGATCAAACTAGAACC TGTTCCAGGATTGCCAGGAGCTCTTCCTCCTCCTCCTGCAGCAGAAGAAGAAGCCTCTGCCAACTACTTC AACTTGCCCCCAAGTGGTCCTCCAGCTGTGGTGAACATTGCTCTGCCACCGCCCCCTGGCATTGCTCCAC CCCCACCCCCAGGTTTTGGGCCACACATGTTCCACCCAATGGGACCACCCCCTCCTTTCATGCGGGCTCC AGGACCAATCCACTATCCTTCTCAGGACCCTCAGAGGATGGGAGCTCATGCTGGAAAACACAGCAGCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC201492 protein sequence Red=Cloning site Green=Tags(s) MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEV CQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKA TSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVA DKLLKRASTMPRLDPPEDKTITTLYVGGLGDTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAA EVAAEKSFNKLIVNGRRLNVKWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYF NLPPSGPPAVVNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_018047 |
| ORF Size | 1260 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018047.3 |
| RefSeq Size | 2352 bp |
| RefSeq ORF | 1263 bp |
| Locus ID | 55696 |
| UniProt ID | Q9NW64 |
| Cytogenetics | 5q33.1 |
| Domains | RRM, zf-CCCH |
| MW | 46.9 kDa |
| Summary | This gene encodes an RNA binding protein. The encoded protein may play a role in cell division and may be involved in pre-mRNA splicing. Related pseudogenes exist on chromosomes 6, 7, 9, 13, 16, 18, and X. [provided by RefSeq, Mar 2009] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC201492L3 | Lenti ORF clone of Human RNA binding motif protein 22 (RBM22), Myc-DDK-tagged | 10 ug | | |
| RC201492L4 | Lenti ORF clone of Human RNA binding motif protein 22 (RBM22), mGFP tagged | 10 ug | | |
| RG201492 | RBM22 (tGFP-tagged) - Human RNA binding motif protein 22 (RBM22) | 10 ug | | |
| SC113736 | RBM22 (untagged)-Human RNA binding motif protein 22 (RBM22) | 10 ug | | |
| SC323783 | RBM22 (untagged)-Human RNA binding motif protein 22 (RBM22) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.