Recombinant 2019-nCov Nucleocapsid Protein
Referencia PR-nCoV-1
embalaje : 100ug
Marca : Novatein Biosciences
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Teléfono : +1 850 650 7790
Recombinant 2019-nCov Nucleocapsid Protein
Data sheet
| Background | N-protein, being one of the predominantly expressed proteins at the early stage of SARS-CoV infection, against which a strong antibody response is initiated by the host; has been proposed to be an attractive diagnostic tool.In serum of SARS-CoV patients, the N-protein has been detected as early as day 1 of infection by ELISA using monoclonal antibodies against it. Furthermore, a comparative study to detect SARS-CoV specific IgG, SARS-CoV RNA, and the N-protein during early stages of infection has demonstrated that detection efficiency of the N-protein is significantly higher than the other two markers. N-protein has been one of the top target for development of IgM ELISA for diagnostic purpose. N-protein has also been tested in its use as a protective vaccine against SARS-CoV infection. So it could be a potential vaccine candidate in the effort to develop an efficient vaccine against 2019-nCov/COVID. |
| Purity | 95%，by SDS-PAGE under reducing conditions. |
| Expression host | E.coli |
| Protein formulation | Lyophilized from 0.22 μm filtered solution in 1x PBS, pH7.4. Contact us for customized product form or formulation. |
| Stability / Shelf Life | The product should be stored at lyophilized state at -20°C or lower. The storage after reconstitution ( avoid repeated freeze-thaw cycles) 2 weeks, 2°C to 8 °C under sterile conditions after reconstitution. 3 months, -20°C under sterile conditions after reconstitution. 12 months, -70°C under sterile conditions after reconstitution. |
| Molecular Weight | The predicted molecular mass is 49 kDa, and the actual MW is about 50 kDa in SDS-PAGE under reducing conditions |
More info
As in other coronaviruses, nucleocapsid (N) is one of the most crucial structural components of the 2019nCoV, or COVID/NCP. Hence major attention has been focused on characterization of this protein. Independent studies conducted by several laboratories have elucidated significant insight into the primary function of this protein, which is to encapsidate the viral genome. The COVID N protein share high homology with the SARS-Cov’s N protein. Therefore, is has very similar function and can have similar applications in the aspect of diagnostic and vaccination.
In infected cells, N protein is mainly located in cytoplasm. Its main function is to package the genomic RNA in a protective covering. In order to achieve this structure, the N-protein must be equipped with two different characteristic properties such as: (i) being able to recognize the genomic RNA and associate with it; (ii) self-associate into an oligomer to form the capsid.
N-protein, being one of the predominantly expressed proteins at the early stage of SARS-CoV infection, against which a strong antibody response is initiated by the host; has been proposed to be an attractive diagnostic tool.In serum of SARS-CoV patients, the N-protein has been detected as early as day 1 of infection by ELISA using monoclonal antibodies against it. Furthermore, a comparative study to detect SARS-CoV specific IgG, SARS-CoV RNA, and the N-protein during early stages of infection has demonstrated that detection efficiency of the N-protein is significantly higher than the other two markers. N-protein has been one of the top target for development of IgM ELISA for diagnostic purpose.
N-protein has also been tested in its use as a protective vaccine against SARS-CoV infection. So it could be a potential vaccine candidate in the effort to develop an efficient vaccine against 2019-nCov/COVID.
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