Safe DNA Gel Stain
Referencia A8743-1ml
embalaje : 1ml
Marca : APExBIO Technology
Contact local distributor :
Teléfono : +1 850 650 7790
- Home
- Superior Choice for Cloning and Genotyping
- Safe DNA Gel Stain
- Home
- Molecular Biology
- DNA Electrophoresis
- DNA Gel Stain
- Safe DNA Gel Stain
Safe DNA Gel Stain
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. It is specifically formulated to be a less hazardous alternative to ethidium bromide (EB) that can be used with either blue-light or UV excitation.
Safe DNA Gel Stain is a better nucleic acid staining reagent for all your molecular biology research needs. It is not only environmental friendly but also good for your sample.
Safe DNA Gel Stain is supplied as 10000X concentrate in DMSO and used in the same way as ethidium bromide solution. It is also suitable for staining RNA in gels.
Cloning efficiency of DNA fragments is improved by using Safe DNA Gel Stain and blue-light, compared with using ethidium bromide and UV light exposure.
- 1. Chin Yee Tan, Danting Jiang, et al. "A commensal-derived sugar protects against metabolic disease." bioRxiv. 2024 Jun 12:2024.06.12.598703. PMID: 38915674
- 2. Ricardo A Meléndez, Daniel T Wynn, et al. "Exploring the role of Casein Kinase 1α Splice Variants Across Cancer Cell Lines." Biochem Biophys Res Commun. 2024 Sep 3:723:150189. PMID: 38852281
- 3. Zhenzhao Sun, Ming Wang, et al. "Getah virus capsid protein undergoes co-condensation with viral genomic RNA to facilitate virion assembly." Int J Biol Macromol. 2024 Apr;265(Pt 1):130847. PMID: 38490381
- 4. Courneya, Isaac Terrance. "Effects of Synonymous and Nonsynonymous CYP51 Mutations on DMI Resistance in Cercospora beticola." North Dakota State University ProQuest Dissertations & Theses, 2024. 31147462.
- 5. Jonathan Molcho, Dana Albagly, et al. "Regulation of early spermatogenesis in the giant prawn Macrobrachium rosenbergii by a GCL homolog." Biol Reprod. 2024 Feb 26:ioae028. PMID: 38408206
- 6. Austin D C Miller, Soham P Chowdhury, et al. "Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types." bioRxiv. 2023 Nov 10:2023.11.07.566095. PMID: 37986852
- 7. Nicolas I E Rocos, Felicity J Coulter, et al. "The minor chicken class I gene BF1 is deleted between short imperfect direct repeats in the B14 and typical B15 major histocompatibility complex (MHC) haplotypes." Immunogenetics. 2023 Oct;75(5):455-464. PMID: 37405420
- 8. Zhichao Tang, Shalakha Hegde, et al. "Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5'untranslated region." bioRxiv. 2023 Oct 23:2023.04.03.535453. PMID: 37066172
- 9. Hannah Sleath, Bortolo Mognetti, et al. "Chemotactic crawling of multivalent vesicles along ligand-density gradients" Cornell University. 16 Oct 2023.
- 10. Wynne, Max Alexander, et al. "Investigation of the structure and function of SecH, a novel component of the Sec machinery in Escherichia coli" University of Birmingham. 2023.
- 11. Daniel Larcombe-Young, Lynsey Whilding, et al. "Generation of human parallel chimeric antigen receptor (pCAR) T cells to achieve synergistic T cell co-stimulation." STAR Protoc. 2022 May 21;3(2):101414. PMID: 35620078
- 12. Joseph Oddy, Rocío Alarcón-Reverte, et al. "Reduced free asparagine in wheat grain resulting from a natural deletion of TaASN-B2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality." BMC Plant Biol. 2021 Jun 29;21(1):302. PMID: 34187359
- 13. Soo Khim Chan, Zhongchao Zhao, et al. "Isolation of a Peptide That Binds toPseudomonas aeruginosaLytic Bacteriophage." ACS Omega 2022, 7, 42, 38053–38060.
- 14. Halle-Wittenberg. "SERF is a modifier of amyloid formation." Martin-Luther-Universität.
- 15. Shen Y, Ha W, et al. "Exome sequencing identifies novel mutation signatures of UV radiation and trichostatin A in primary human keratinocytes." Sci Rep. 2020;10(1):4943. PMID: 32188867
- 16. Urrutia G, Salmonson A, et al. "Combined Targeting of G9a and Checkpoint kinase 1 Synergistically Inhibits Pancreatic Cancer Cell Growth by Replication Fork Collapse." Mol Cancer Res. 2019 Dec 10. pii: molcanres.0490.2019. PMID: 31822519
- 17. Volkman HE, Cambier S, et al. "Tight nuclear tethering of cGAS is essential for preventing autoreactivity." Elife. 2019 Dec 6;8. pii: e47491. PMID: 31808743
- 18. Hannah E Volkman, Stephanie Cambier, et al. "cGAS is predominantly a nuclear protein." bioRxiv. 2018 December 04.
• Reduce exposure to highly mutagenic EB and harmful UV light.
Safe DNA Gel Stain is less mutagenic and safer for you to work with than ethidium bromide. You can further decrease your exposure risk by using visible blue-light with Safe stain rather than UV. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.
• Increase sensitivity by reducing nonspecific background fluorescence.
Safe DNA Gel Stain offers excellent sensitivity in nucleic acid visualization with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. Plus, when used with blue light, Safe DNA Gel Stain has less background fluorescence than ethidium bromide–stained gels with UV light.
• Alternative to EB for all staining applications, including RNA staining.
Safe DNA Gel Stain is supplied as a concentrate in DMSO which can be used in the same way as ethidium bromide. It can be added into an agarose gel for staining during electrophoresis at a ratio of 1: 10000 or apply it after electrophoresis at a ratio of 1: 3300. Store at room temperature in its original packaging to avoid excessive light exposure.
Note: Safe DNA Gel Stain is less efficient in visualizing low molecule weight bands (100-200 bp).
| Physical Appearance | DMSO solution |
| Storage | Store at 4°C, protect from light for 6 months. |
| M.Wt | 504.66 |
| Formula | C28H28N2O3S2 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥14.67 mg/mL in DMSO |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |