SUMO protease, also known as Ulp, is a highly active cysteine protease derived from a recombinant gene fragment of Ubl-specific protease 1 in Saccharomyces cerevisiae. SUMO proteases recognize and cleave peptide bonds after the SUMO carboxyl (C-terminal) of the Ubiquitin-like (UBL) protein in a highly specific manner. SUMO is an Ubiquitin-like protein that is commonly found in post-translation modification (PTM), which plays an important role in the stability of proteins and the regulation of biological functions.
The optimal lysis temperature for SUMO proteases is 30 , but it is in the wide pH range (6.0-10.0) and temperature range (2 to 30 -400mM NaCl) maintains high enzyme activity. In special scenario use, the sample can be digested overnight at 4~2mM), and the addition of DTT at the appropriate concentration to the digestion system can significantly improve the digestion efficiency, especially during long-term digestion, such as Enzymes are cut overnight at 4 >Quality Control