TransDetect^® In Situ Click TUNEL Imaging Kit-555 Fluorophore is a TUNEL assay reagent based on the Click reaction. In apoptosis, double-strand breaks or single strand breaks of genomic DNA can produce a large number of sticky 3'-OH ends. EdUTP is a dUTP nucleotide modified with a small alkyne moiety, which is more readily incorporated at the 3´-OH ends of fragmented DNA by the Terminal deoxynucleotidyl transferase (TdT) enzyme. A covalent reaction occurs between the azide and the alkyne moiety on EdUTP based on the Click reaction, and more sensitive TUNEL detection can be performed via fluorophore on the azide.

Storage

at -20˚C in the dark for one year

Shipping

Dry ice (-70℃)