Tris-Glycine-SDS Powder (1 L of 1×)
Referencia HY-K1020-10
embalaje : 10pouches
Marca : MedChemExpress
MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot.
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Description & Advantages |
Tris-Glycine-SDS (TGS) is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot. MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS. Each pouch can be diluted to 1 L 1× TGS buffer, in which the concentration of Tris is 25 mM, the concentration of Glycine is 192 mM and the concentration of SDS is 0.1%. This product provides a convenient way to make TGS buffer and eliminates the need to weigh and mix individual components. |
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Almacenamiento |
Store at room temperature for 3 years. |
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Protocolo |
1. Add 1 pouch of Tris-Glycine-SDS Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer. 2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TGS buffer. Note: The pH of the 1× TGS buffer is 8.3 ± 0.2 @ 25°C. |
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Componentes |
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Documentación |
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.