TS1R1 Human Recombinant Protein, Synthetic Nanodisc

Referencia TP721854M

embalaje : 100ug

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TS1R1 Human Recombinant Protein, Synthetic Nanodisc

Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
C-terminal Flag tagged overexpression cDNA clone
Tag C-term Flag Tag
Predicted MW The human full length TS1R1 protein has a MW of 93.1kDa
Concentration Please refer to the Certificate of Analysis (COA) for the lot-specific concentration before lyophilization.
Purity The purity of the protein is determined by SDS-PAGE and Coomassie blue staining.
Buffer Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Do not use solvents with pH lower than 6.5 in subsequent experiments.
Reconstitution Method
  1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
  2. Calculate the volume for reconstitution (in µL) using the formula: [Quantity (mg)/Concentration (mg/mL)]x1000
  3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
  4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Preparation Affinity purification
Storage Store at -20°C to -80°C for 12 months in lyophilized form.
Stability After reconstitution, if not intended for use within a month, aliquot and store at -80°C . Avoid repeated freezing and thawing.
Reference Data
Locus ID 80835
UniProt ID Q7RTX1
Synonyms GM148, GPR70, T1R1, TR1
Protein Families Druggable Genome, Transmembrane
Protein Pathways Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway
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