HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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RUOCE / IVD
- Unconjugated 995
- HRP 103
- FITC 66
- Biotin 64
- PE 54
- APC 49
- MaxLight 490 31
- MaxLight 550 31
- MaxLight 650 31
- MaxLight 405 18
- MaxLight 750 18
- Cy3 14
- Alexa Fluor 350 8
- Alexa Fluor 488 8
- Alexa Fluor 555 8
- Alexa Fluor 750 8
- Cy5 8
- Cy5.5 8
- Cy7 8
- APC-CY5.5 1
- APC-Cy7 1
- Alexa Fluor 405 1
- Alexa Fluor 594 1
- Alexa Fluor 647 1
- Alexa Fluor 680 1
- PE-Cy5 1
- PE-Cy5.5 1
- PE-Cy7 1
- PerCP-Cy5.5 1
- PerCp 1
- RBITC 1
- human 1034
- mouse 337
- rat 304
- cow 50
- e. coli 49
- dog 47
- pig 47
- cat 26
- rabbit 24
- horse 23
- monkey 19
- sheep 18
- camel 16
- hamster 16
- guinea pig 7
- chicken 6
- plant 5
- donkey 4
- duck 4
- fish 4
- goose 4
- pigeon 4
- xenopus 2
- zebrafish 2
- rabbit 570
- mouse 429
- human 347
- e. coli 128
- yeast 122
- 48
- rat 40
- goat 36
- horse 12
- dog 10
- hamster 10
- sheep 10
- virus 9
- cow 8
- monkey 8
- pig 8
- bird 5
- chicken 5
- cat 1
- donkey 1
- guinea pig 1
- Primary antibody 1172
- Protein/peptide 973
- Biochemicals 669
- ELISA/ELISPOT 354
- Inhibitor/Antagonist/Agonist 277
- CRISPR/Cas9 149
- Buffers and reagents 147
- RNAi 100
- Destination vector 50
- kit 34
- Other products 22
- Secondary antibody 12
- Cell line 6
- Probe 6
- cDNA Clone 4
- Lysate 2
- Paraffin tissue section 1
- Primer 1
- ELISA 717
- WB 707
- IHC 423
- FLISA 121
- IP 95
- ICC 87
- FC 82
- IF 75
- Enzyme-linked immunosorbent assay for Antigen Detection. 10
- 0.078 ng/mL 3
- Chemiluminescence 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- PCR 1
- Polyclonal 615
- 2G6 31
- 4G5 31
- 1F8 30
- 1G1 30
- 1G5 30
- 10i46 25
- 13H261 21
- 3H1166 21
- Monoclonal 14
- M-A261 12
- HLy9.1.25 8
- 1A10A10 5
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
- 13.2G8 4
- 13.6D9 4
- 13.7E2 4
- 13.7H4 4
- 13.8E5 4
- 13B4.E10 4
- 14.2H9 4
- 14F10.H9 4
- 1A6 4
- 26.7C4 4
- 2E4 4
- 3G8 4
- 4A8 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- 1626CT702.263.21 3
- 6D23 3
- SAA1697 3
- 12K274 2
- 17D17 2
- 17L18 2
- 202.36 2
- 2213 2
- 2M2 2
- 3F9-2C2 2
- 5B20 2
- 7H111 2
- 8C10 2
- B2M-01 2
- B2M-02 2
- RB21227 2
- 11C257 1
- 12K99 1
- 134-2C2 1
- 13B822 1
- 5K78 1
- 5K78 (134-2C2) 1
- 6C1 1
- 6C5 1
- 9H177 1
- ADAB-8 1
- DAB-6 1
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