SYBR Green qPCR Mix with high ROX
SYBR Green High ROX quantitative PCR mixes are made for use with qPCR devices requiring high ROX as an internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations.
The SYBR Green mix consists of all the necessary components (except template DNA and primers): a Taq polymerase, dNTPs, MgCl2, SYBR Green and High ROX dye.
Here's how to select the appropriate ROX dye for the qPCR device you are using:
| Universal | All instruments |
| Without ROX | Bio-Rad
CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2,
Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
| Low ROX | Applied
Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P |
| High ROX | Applied
Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus |
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