Assay Principle: 

The DetectX® Arg8-Vasopressin (AVP) Chemiluminescent ELISA Kit quantitatively measures AVP in extracted serum, extracted plasma, and tissue culture media. This kit is a competitive ELISA with a run time of 16 hours. Please read the complete kit insert for more information before performing this assay. Use the provided AVP standard to generate a standard curve for the assay.

Protocol Summary:

• Transfer standards or diluted samples into the included transparent microtiter plate coated with goat anti-rabbit IgG antibody.
• Add AVP peroxidase conjugate and AVP polyclonal rabbit antibody, and incubate the mixture at 4°C with shaking for 16 hours. The immunological reaction varies inversely with the AVP concentration in the sample.
• Post-incubation, remove excess conjugate and introduce the chemiluminescent substrate. The substrate reacts with the bound conjugate, producing a detectable light signal.
• Calculate AVP concentration using a plate reader, based on the standard curve.

Background:

Arg8-Vasopressin (AVP) is a key hormone involved in body water regulation and osmotic balance. The posterior pituitary gland releases AVP in response to factors like sexual stimulation, uterine dilatation, stress, and dehydration, primarily acting on renal collecting tubules to increase water reabsorption. AVP imbalances are central to conditions such as diabetes insipidus, which involves the inability to concentrate urine adequately, often due to decreased AVP production or renal response.

Inappropriate AVP secretion is commonly observed in elderly patients and those with central nervous system pathologies, including head injuries, strokes, or cerebral tumors. Furthermore, AVP plays a important role in social behavior, sexual motivation, and pair bonding. Its release into the brain influences maternal responses to stress and various aspects of interpersonal relationships.

The DetectX® Arg8-Vasopressin (AVP) Chemiluminescent ELISA Kit is an important tool for endocrinology and neurobiology research, facilitating the study of AVP’s multifaceted roles in human physiology and pathology. This kit ensures high sensitivity and specificity for detailed hormonal analysis in diverse biological samples.