Aspartate BioAssay™ Kit

Aspartate, a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis. The Aspartate BioAssay™ Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570nm or fluorescence intensity at lex/em =530/585nm is directly proportional to the aspartate concentration in the sample.||Intended Use:|For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.||Applications:|Direct Assays: aspartate in plasma, serum, tissue and culture media.|Drug Discovery/Pharmacology: effects of drugs on aspartate metabolism.||Range:|2-400um aspartate for colorimetric assays and 1-50um for fluorometric assays.||Detection limit: |OD, FL: 2, 1um||Kit Components:|Developer, 1x1ml|Dye Reagent, 1x120ul|ODC Enzyme, 1x120ul|AST Enzyme, 1x240ul|Cosubstrate, 1x600ul|Aspartate Standard, 1x400ul||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.