Human FADD antibody
Referencia AFA0901-100
embalaje : 100ul
Marca : NKMAX BIO
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Teléfono : +1 850 650 7790
ICC/IF analysis of FADD in HeLa cells. The cell was stained with AFA0901 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates of Jurkat (30ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : Raw264.7 cell lysate
Lane 3. : MCF7 cell lysate
Lane 4. : A431 cell lysate
Lane 5. : Ramos cell lysate
Lane 6. : Raji cell lysate
Lane 7. : Balb/3T3 cell lysate
Lane 1. : HeLa cell lysate
Lane 2. : Raw264.7 cell lysate
Lane 3. : MCF7 cell lysate
Lane 4. : A431 cell lysate
Lane 5. : Ramos cell lysate
Lane 6. : Raji cell lysate
Lane 7. : Balb/3T3 cell lysate
Immunohistochemistry (IHC)
Paraffin embedded sections of human breast cancer tissue were incubated with anti-human FADD (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.