Human S100 calcium binding protein B (S-100B) ELISA Kit
Referencia KTE60758-96
embalaje : 96T
Marca : Abbkine
Specification
Product name | Human S100 calcium binding protein B (S-100B) ELISA Kit |
Reactivity | Human |
Applications | ELISA |
Applications notes | This Human S100 calcium binding protein B (S-100B) ELISA Kit employs a two-site sandwich ELISA to quantitate S-100B in samples. An antibody specific for S-100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS-100B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S-100B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S-100B bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method | Colorimetric |
SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
Assay type | Sandwich ELISA (quantitative) |
Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Alternative | S-100B; NEF; S100; S100beta; OTTHUMP00000174958; S-100 calcium-binding protein; beta chain; S100 beta; S100 calcium binding protein; beta (neural); S100 calcium-binding protein; beta; S100 calcium-binding protei |
Product Properties
Kit components | calcium binding protein B microplate calcium binding protein B standard calcium binding protein B detect antibody calcium binding protein B (S-100B) ELISA Kit has high sensitivity and excellent specificity for detection of Human S-100B. No significant cross-reactivity or interference between Human S-100B and analogues was observed. |
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Calibration range | Please inquire | ||||||||
Limit of detection | Please inquire | ||||||||
Usage notes | minutes before opening. minutes. - 8 class="pro-form-title">Additional Information
Image & description |