96 Tests PN: C280010

32 Tests PN: C280010T

Components:

1x Ab-conjugated beads (premixed).

2x Biotin-detection Ab (premixed).

2x Mouse/Rat dAb Diluent.

Lyophilized Standard Mix- Mouse TH1/Th2/Th17 10-Plex.

96 & 32 Tests PN: MG28010. One vial containing lyophilized recombinant GM-CSF, IFNγ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-10, IL-17A and TNFα.

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels: 

  • 10x Reading Buffer. 

  • 10x Wash Buffer. 

  • PCR 8-tube Strip. 

  • Filter Plate and Lid. 

  • Plate Seals. 

    • Application: Optimal antibody pairs and antigen standards for assaying the mouse Th1/Th2/Th17 10-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005), 32 Tests (PN: U100005T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure. 

    Storage: 2-8 C in the dark.

    Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

    Assay Specifications:

    • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

    • Sensitivity (LODs): GM-CSF, < 5 pg/mL; IFNγ, < 0.5 pg/mL; IL-1α, < 2 pg/mL; IL-2, < 2 pg/mL; IL-4, < 0.5 pg/mL; IL-5, < 5 pg/mL; IL-6, < 3 pg/mL; IL-10, < 2 pg/mL; IL-17A, <1.5 pg/mL; TNFα, < 0.5 pg/mL.

    • LLOQs: GM-CSF, < 10 pg/mL; IFNγ, < 2 pg/mL; IL-1α, < 10 pg/mL; IL-2, < 5 pg/mL; IL-4, < 2 pg/mL; IL-5, < 10 pg/mL; IL-6, < 10 pg/mL;  IL-10, < 5 pg/mL; IL-17A, < 5 pg/mL; TNFα, < 2 pg/mL.

    • ULOQs: GM-CSF, > 5,000 pg/mL; IFNγ, > 5,000 pg/mL; IL-1α, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-5, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-17A, > 5,000 pg/mL; TNFα, > 1,500 pg/mL.

    • Standard dose recovery: 70-130%

    • Intra-assay CV: < 10%

    • Inter-assay CV: < 20%

    • Cross-reactivity of analytes in the Panel: Negligible

    • Sample volume: 15 columns-12 empty" data-layout-label="Mouse Th1/Th2/Th17 10-plex (96 & 32 Tests) Footer Content" data-type="block-field" id="page-footer-590fbad3b8a79b2147afcbd2">