NUF2 (NM_145697) Human Recombinant Protein

CAT#: TP760784

Purified recombinant protein of Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug


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Specifications

Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence encoding human full-length NUF2
Tag N-His
Predicted MW 54.1 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq NP_663735
Locus ID 83540
UniProt ID Q9BZD4
Cytogenetics 1q23.3
Refseq Size 2003
Refseq ORF 1392
Synonyms CDCA1; CT106; NUF2R
Summary This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome

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