Product Description  

One Step Mouse Genotyping Kit is specially designed for the rapid genotyping of mouse, which contains a complete set of reagents for DNA extraction and PCR amplification. This kit can be used for the rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification with no need of homogenization, crushing, overnight digestion, phenol-chloroform extraction, DNA precipitation or column purification operations, which greatly shortens the experimental time. During use, immerse the tissue into a lysis solution premixed with Proteinase K, incubate at 55℃ for 20 min, and then heat at 95℃ for 5 min to inactivate Proteinase K. After centrifugation, the lysate can be directly used as the template for PCR amplification. After repeated tests, it is widely applicable to the amplification of target fragments within 2 kb, and is suitable for Multiplex PCR within four fragments.

 Features  

• Mouse-Direct PCR: No need for DNA purification steps; crude Samples can be added directly to PCR

• Convenient operation: Master Mix format with premixed gel loading dye; reduces sample handling and allows direct loading on gel

• Special optimization: Lysis solution based on proteinase K; suitable for rapid genotyping of mouse

• Broad compatibility: Suitable for ≤ 2kb fragments; up to 4-plex amplification

 Components  

 Storage  

1 × Mouse tissue Lysis Buffer, store at 2 ~ 8°C;

Other components, store at -30 ~ -15°C;

Transport at ≤0°C.