Assay Principle:

The DetectX® Progesterone ELISA Kit provides an efficient method for quantitatively measuring progesterone in serum, plasma, fecal extracts, urine, and tissue culture media. This competitive ELISA, with a run time of 2.5 hours, offers a balance of precision and efficiency. Read the kit insert for detailed instructions before starting the assay. The kit includes a progesterone standard to create an accurate standard curve.

Protocol Summary:

• Add standards or diluted samples to the provided transparent microtiter plate coated with goat anti-mouse IgG antibody.
• Introduce progesterone peroxidase conjugate and progesterone monoclonal mouse antibody to initiate the immunological reaction.
• Incubate the mixture covered at room temperature with shaking for 2 hours. The reaction varies inversely with progesterone concentration in the sample.
• After incubation, wash away excess conjugate and add the TMB substrate. TMB substrate reacts with the bound conjugate to produce a detectable signal.
• Use a plate reader to measure the signal at 450nm and calculate progesterone concentration using the standard curve.

Background:

Progesterone, also known as P4 (Pregn-4-ene-3,20-dione), is a C21 steroid hormone integral to the female menstrual cycle, gestation, and embryogenesis in humans and other species. It is the main naturally occurring hormone in the class of hormones called progestogens.

Progesterone plays essential roles in regulating human female reproductive function, impacting the uterus, ovary, mammary gland, and brain. It also has significant functions in non-reproductive tissues, including the cardiovascular, skeletal, and central nervous systems. Progesterone’s actions are mediated through two isoforms of the nuclear progesterone receptor (PR), PR-A and PR-B. These isoforms are found in various healthy and cancerous breast tissue cells in humans, rats, and mice. Additionally, progesterone has neurotrophic roles in the peripheral nervous system, promoting the growth and maturation of axons and stimulating the repair of myelin sheaths in regenerating nerve fibers. The DetectX® Progesterone ELISA Kit is a valuable tool for researchers and clinicians studying reproductive physiology and progesterone’s wider roles in the body.