Rat Anti-Mouse CD8α-SPRD
Referencia 1550-13
embalaje : 0.1mg
Marca : Southern Biotech
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Teléfono : +1 850 650 7790
| Clone | 53-6.7 |
|---|---|
| Isotype | Rat (LOU/Ws1/M) IgG2aκ |
| Isotype Control | Rat IgG2a-SPRD (KLH/G2a-1-1) |
| Specificity | Mouse CD8α |
| Alternative Names | Ly-2, Lyt-2, Ly-35, Ly-B, T8 |
| Description | In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. |
| Immunogen | Spleen cells or thymocyte membranes |
| Conjugate | SPRD (R-phycoerythrin/Cyanine 5) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Trademark Information | Spectral Red® is a registered trademark of Southern Biotechnology Associates, Inc. Spectral Red® is a PE/CY5 tandem conjugate Cy® is a registered trademark of Global Life Sciences Solutions Germany GmbH |
| Applications | Flow Cytometry – Quality tested 1,6,7,9-15 Immunohistochemistry-Frozen Sections – Reported in literature 2-5 Immunocytochemistry – Reported in literature 7 Immunoprecipitation – Reported in literature 1,6 Depletion – Reported in literature 8 Blocking – Reported in literature 6 |
| RRID Number | AB_2794878 |
| Gene ID | 12525 (Mouse) |
| Gene ID Symbol | Cd8a (Mouse) |
| Gene ID Aliases | Ly-2; Ly-B; Ly-35; Lyt-2; BB154331 |
| UniProt ID | P01731 (Mouse |
| UniProt Name | CD8A_MOUSE (Mouse) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 2. Konno A, Takada K, Saegusa J, Takiguchi M. Presence of B7-2+ dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjörgren's syndrome. Autoimmunity. 2003;36:247-54. (IHC-FS)
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