SK-N-SH cells
Referencia C0005006
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Disease:
Neuroblastoma
Origin:
Brain; derived from metastatic site: bone marrow. The SK-N-SH line differs from SK-N-MC in that it exhibits a longer doubling time and higher levels of dopamine - beta - hydroxylase.
Species:
Homo sapiens Brain Epithelial Adherent 2n = 46, modal no. 47 Female, Caucasian, 4 years of age AddexBio-formulated EMEM (C0005-01) + 10% FBS When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; seed at 3x10^4 cells/cm2, feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Complete culture medium supplemented with 5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 8,13 D5S818: 12 D7S820: 7,10 THO1: 7,10 TPOX: 8,11 vWA: 14,18 If use of this culture results in a scientific publication, it should be cited in the publication as: SK-N-SH (AddexBio C0005006) Tissue:
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Citation Format:
References for SK-N-SH cells:
1. Gilbert LC, Wachsman JT. Characterization and partial purification of the plasminogen activator from human neuroblastoma cell line, SK-N-SH. A comparison with human urokinase. Biochim Biophys Acta. 1982 Jun 24;704(3):450-460. PMID: 7052133