Flow Cytometry: Use 10 μl of 1/10-1/25 diluted antibody to label 106 cells or 100 μl whole blood. Immunoprecipitation. Western Blot. Functional Assays.
Reactivity
Canine, Guinea Pig, Human, Monkey, Porcine
Antibody Host
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity
This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS.
TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
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