UltraGRO™ Research grade
Product Type:hPL
Attribute:Xeno-free
Grade:Research grade
Product Name:UltraGRO™
Catalog Number:HPCPLCRL50, HPCPLCRL10, HPCPLCRL05
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Description:
UltraGRO™ cell culture supplement is a xeno-free, animal serum-free, and heparin-requiring supplement for replacing FBS (fetal bovine serum) to support cell expansion from research use. UltraGRO™ contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale production.
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Quality Control Test:
1. Appearance: Yellow to Buff liquid
2. Total Protein: 4.0 - 8.0 g/dL
3. Endotoxin: ≤ 10 EU/mL
4. Sterility: Pass
5. Mycoplasma: Negative
6. pH: Tested
7. Osmolality: Tested
8. Performance Test: Test and record
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Storage and Shipping Information:
UltraGRO™ is most stable when stored frozen until needed. The recommended storage temperature is -20°C. Thaw frozen UltraGRO™ product in 37°C water bath before use. Once UltraGRO™ is thawed, it is recommended to fully use for completed medium preparation (e.g. 5%) the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C.
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Packaging Information:
500mL, 100mL, 50mL plastic bottles
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Media Form:
Frozen Liquid
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Application:
For human ex-vivo tissue and cell culture processing applications.
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Instructions for Use:
1. UltraGRO™ shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.
2. We recommend seeding MSCs at approximately 3x103~6x103 per cm2.
3. UltraGRO™ requires heparin at a final concentration of 2 IU/mL to be added in the culture media when supplemented with 5% UltraGRO™. Failure to add heparin will result in coagulation during cell culture in typical media.
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Cell Lines:
bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells.
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Important Information:
1. Clotting or insoluble particles may form in thawed UltraGRO™. Published research has shown that particles will not alter the performance of the product.
2. Clotting or insoluble particles may form in thawed UltraGRO™, it is recommended to centrifuge at 3,400 xg for 3~5 minutes or to filter the liquid concentrate with a sterile 40 μm Cell Strainer to remove insoluble particles.
3. Filtering the completed medium (e.g. 5%), after UltraGRO™ is diluted in the basal medium, will not affect UltraGRO™ supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™ concentrate, as this may reduce 5% UltraGRO™ cell culture performance.
4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™ performance.
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Safety information:
1. Follow the handling instructions outlined in the Material Safety Data Sheets (MSDSs). Wear appropriate protective eyewear, clothing, and gloves.
2. UltraGRO™ is a cell culture supplement derived from human platelets collected from healthy donors at FDA or HC licensed blood centers. Each donor has been tested using FDA/HC-licensed tests and found nonreactive for HBsAg, Hepatitis B core antibody (anti-HBc), HIV antibody (anti-HIV-1/2), Hepatitis C antibody (anti-HCV), HTLV-1/2 antibody (anti-HTLV-1/2), Trypanosoma cruzi antibody (anti-T. cruzi), HIV-1, HCV, HBV, WNV nucleic acid testing and Syphilis microhemagglutination test.
3. This product is for in vitro use only.