General Info
| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Human/Monkey/Pig/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-BGLAP (50-100) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Purification: | Affinity Purified |
| Concentration: | 0.55-0.75 µg/µl |
| Dilution Range: | WB: 1:500ELISA: 1:10, 000IP: 1:150IHC: 1:100 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Information
| Gene Symbol: | BGLAP |
| Gene ID: | 632 |
| Uniprot ID: | OSTCN_HUMAN |
| Immunogen Region: | 50-100 |
| Immunogen: | Synthetic peptide taken within amino acid region 50-100 on human Osteocalcin preprotein. |
Description
| Post Translational Modifications | Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation by GGCX. These residues are essential for the binding of calcium . Decarboxylation promotes the hormone activity. |
| Function | Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation. Functions to limit bone formation without impairing bone resorption or mineralization. It binds strongly to apatite and calcium. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. |
| Protein Name | OsteocalcinBone Gla ProteinBgpGamma-Carboxyglutamic Acid-Containing Protein |
| Database Links | Reactome: R-HSA-159740Reactome: R-HSA-159763Reactome: R-HSA-159782Reactome: R-HSA-8940973 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Osteocalcin antibodyAnti-Bone Gla Protein antibodyAnti-Bgp antibodyAnti-Gamma-Carboxyglutamic Acid-Containing Protein antibodyAnti-BGLAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance