Anti-GOAT IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated
Referência 605-703-002
Tamanho : 2mg
Marca : Rockland Immunochemicals
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Telefone : +1 850 650 7790
Specifications for Goat IgG (H&L) Antibody Peroxidase Conjugated
Product Details
Donkey Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated - 605-703-002
Donkey anti-Goat IgG Antibody Peroxidase conjugation, Donkey anti-Goat IgG HRP conjugated antibody
Donkey
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Goat
Native Protein
Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum.
Application Details
ELISA
WB - View References
Anti-Goat IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. It is also suitable in IHC. Specific conditions for reactivity and signal detection should be optimized by the end user.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Anti-Goat IgG Peroxidase Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
References (4)
Guo Y et al. (2021). p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway. Cell Death Dis.
Applications
WB, IB, PCA PubMed
Monette A. et al. (2020). Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell Rep.
Applications
WB, IB, PCA PubMed
Tarassishin L et al. (2014). Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. Journal of Proteomics
Applications
WB, IB, PCA PubMed
Gauthamadasa K et al. (2012). Apolipoprotein A-II-mediated conformational changes of apolipoprotein A-I in discoidal high density lipoproteins. J Biol Chem.
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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