Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction. Sandwich ELISA using RM117 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. ELISA showing RM117 reacts only to human IgG1, and not to any other IgG subclasses (IgG2, IgG3, or IgG4), and no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG binding antibody (positive control) was used as the detection antibody. Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody, Clone RM117, at a 1:15,000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA
Product Details Product Name Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117 Product Description Rabbit monoclonal to Human IgG1 Catalog No. 31-1019-00 Clone Name RM117 Product Data Sheet Anti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117 Specificity RM117 reacts to the heavy chain of human IgG1. RM117 does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM117 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. Immunogen Peptide corresponding to the hinge region of Human IgG1 Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA Species Reactivity Human Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Flow Cytometry (FC): 2 µg/mL - 8 µg/mL.
References for Human IgG1 antibody [RM117] Harel E et al. Further Evidence That the Soluble Urokinase Plasminogen Activator Receptor Does Not Directly Injure Mice or Human Podocytes. J Transplantation. (2020), 10.1097/TP.0000000000002930. IHC; Human. Read more (PubMed: 31449183)
Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305) . Product Images
Anti-Human IgG1 rabbit monoclonal antibody [RM117]
![Anti-Human IgG1 rabbit monoclonal antibody [RM117]](https://www.quimigen.pt/upload/-img-lapin1.png)
Referência 31-1019-00
Tamanho : 100ug
Marca : RevMab Biosciences
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Rabbit monoclonal to Human IgG1
100 µg
