Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) is a combination of the DMEM and F-12 media. It is widely used fo mammalian cellculture. It contains a high concentration of glucose along with various amino acids, vitamins and inorganic salts essential for cell growth, though does not contain proteins, lipids or growth factors.

The 1 × 10 L size is provided as 1 bottle containing lyophilized powder for 10 L of 1X DMEM/F-12. The 1 × 50 L size is provided as 1 bottle containing lyophilized powder for 50 L of 1X DMEM/F-12.

Target	DMEM/F12
Form	Lyophilized
Reconstitution	Reconstitute in distilled water.
Storage	Store between 2-8 °C. Avoid exposure to light.
Validity	Up to 36 months.
Buffer	After reconstitution: pH 7.2-7.4, containing 3151 mg/L D-glucose, 2.5 mM L-glutamine, 8.1 mg/L phenol red and 0.5 mM sodium pyruvate. Does not contain HEPES or NaHCO3.
Endotoxin Level	< 1 EU/ml
Concentration	12.04 g/L
Availability	Shipped within 5-7 working days.
Note	This product is for research use only.
Directions for use	This product should be used with serum (or equivalent) supplementation. Use aseptic techniques to avoid contamination.

Research Articles on DMEM/F12