GPT ELISA Kit (Human)
Referência OKEH00299
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
| Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology GPT ELISA Kit (Human) (OKEH00299) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for GPT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPT captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.2% (n = 20) Mean Inter-assay CV%: < 6.8% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.078 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Alanine aminotransferase 1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | GPT |
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| Gene Full Name | glutamic--pyruvic transaminase |
| Alias Symbols | AAT1;alanine aminotransferase;alanine aminotransferase 1;alanine transaminase;ALT1;glutamate pyruvate transaminase 1;glutamic-alanine transaminase 1;Glutamic--alanine transaminase 1;glutamic-pyruvate transaminase (alanine aminotransferase);glutamic--pyruvic transaminase 1;GPT1. |
| NCBI Gene Id | 2875 |
| Protein Name | P24298 |
| Description of Target | Alanine aminotransferase 1 |
| Uniprot ID | https://www.uniprot.org/uniprot/P24298 |
| Protein Accession # | NP_005300.1 |
| Nucleotide Accession # | NM_005309.2 |
| Protein Size (# AA) | 496 |
| Molecular Weight | 55 kDa |