600170 | Human SCF Quimigen Portugal

Recombinant Active Protein

Cat. No.

600170

Unit

0.01 mg

Description

ReStem Cell Factor (SCF) stimulates the proliferation of mast cells, augments the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture, and mediates cell-cell adhesion. SCF acts synergistically with other cytokines such as interleukins. Recombinant Human SCF produced in E. coli is a single non-glycosylated polypeptide chain containing 165 amino acids with a MW of 18,409 Da.

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Datasheet

Format

Each vial contains 10 ug protein lyophilized in 10 mM acetic acid. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

Kit ligand; C-kit ligand; stem cell factor; SCF; mast cell growth factor; MGF; KITLG

Accession No.

P21583

18409 Da

Sequence

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVALPPVA

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Human SCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is less then 1.7 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Human SCF

Referência 600170

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Marca : Abbiotec

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