IL-5, Anti-Human
Referência NB-50-0926-1
Tamanho : 1mg
Marca : Neo Biotech
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Telefone : +1 850 650 7790
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Western Blot: To detect Human IL-5 by Western Blot analysis IL-5 Polyclonal Antibody (Product # 500-P25-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot: To detect Human IL-5 by Western Blot analysis IL-5 Polyclonal Antibody (Product # 500-P25-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA: To detect Human IL-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-5 Polyclonal Antibody (Product # 500-P25-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-5 Polyclonal Antibody, Biotin (Product # 500-P25BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-5.
Product Details
500-P25-100UG
Species Reactivity
Published species
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Immunogen
Conjugate
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Product Specific Information
AA Sequence of recombinant protein: MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-5. Anti-Human IL-5-specific antibody was purified by affinity chromatography employing an immobilized Human IL-5 matrix.
Sandwich ELISA: To detect Human IL-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-5 (500-P25Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-5.
Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell differentiation factor I; colony-stimulating factor, eosinophil; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: EDF; IL-5; IL5; TRF