Mouse IgM Antibody : APC
Referência OASB00183
Tamanho : 0.1mg
Marca : Aviva Systems Biology
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Mouse IgM Antibody : APC (OASB00183)
| Datasheets/Manuals | Printable datasheet for Mouse IgM Antibody : APC (OASB00183) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 1B4B1 |
| Isotype | IgG1 |
| Host | Rat |
| Conjugation | APC |
| Application | FC, FLISA |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | 0.1 mg/mL |
| Specificity | IgM |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of astandard reference reagent using immunofluorescence staining. Representative data are included in this datasheet. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: ELISA: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate R-phycoerythrin-Cyanine 7 conjugate Alkaline phosphatase conjugate Horseradish peroxidase conjugate <=1 ug/106 cells <=1 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells 1:1,000-1:2,000 1:2,000-1:4,000 |
| Application Info | Flow cytometry 1, In vitro T cell activation 1,5,6,9, Induction of apoptosis 4,7 |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs. |
| Other Applications Image 1 Data | Amount Used: 0.3 Ug/106 cells Cells from BALB/c spleen were doublestained with rat anti-mouse IgM-R-PE and rat anti-mouse CD19-FITC , following which small lymphocytes were gated and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 anda stabilizing agent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
