Rabbit Anti-Donkey IgG Secondary Antibody (Biotin)
Referência LS-C61192-2
Tamanho : 2mg
Marca : LSBio
Overview
Antibody:
Donkey IgG Rabbit anti-Donkey Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Donkey
Format:
Biotin, Unmodified
Specifications
Description
Donkey IgG antibody LS-C61192 is a biotin-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Donkey IgG
Host
Rabbit
Reactivity
Donkey (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Donkey IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Donkey IgG and Donkey Serum.
Applications
- IHC (1:1000 - 1:5000)
- Western blot (1:2000 - 1:10000)
- ELISA (1:20000 - 1:100000)
Usage
Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@quimigen.pt