Western blot analysis of Collagen 1 alpha 1 expression in EC9706 (A), A375 (B), SGC7901 (C), Hela (D), HEK293T (E) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 130 kD)

Immunofluorescent analysis of Collagen 1 alpha 1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).