Rat Interleukin-1 beta Recombinant Protein
Referência 012-001-301-0100
Tamanho : 100ug
Marca : Rockland Immunochemicals
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Specifications for rRat IL-1 beta Protein
Product Details
Rat Interleukin-1 beta Recombinant Protein - 012-001-301-0010
Catabolin, LAF, EP, LEM, MCF
Rat
E. coli
Recombinant Protein
Target Details
Il1b - View All Il1b Products
Interleukin-1 beta purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
Q63264 - UniProtKB
Application Details
SDS-PAGE
Cellular Assay - View References
Interleukin-1-beta Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-1-beta in immunological assays.
Formulation
0.01 M Sodium Phosphate, pH 7.5
None
None
10µl (10-100µl)
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Expiration date is six (6) months from date of receipt.
Background
Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine, produced in response to inflammatory agents by a variety of cells, including, monocytes, macrophages, and dendritic cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant rat IL-1β is a non-glycosylated protein, containing 152 amino acids, with a molecular weight of 17.3 kDa.
Related Protocols
Azide Removal by Dialysis Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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Literature
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Qualification & Validation
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