Recombinant Protein Human IFN-gamma R1 - Fc Chimera

Symansis' IFN-gamma R1 - Fc^hcx Chimera has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F. Symansis' IFN-gamma R1 - Fc^hcx Chimera contains 10-35% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Oritani et al. (2001) Cytokine Growth Factor Rev. 12(4):337-48