A8743-1ml | Safe DNA Gel Stain Quimigen Portugal

Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. It is specifically formulated to be a less hazardous alternative to ethidium bromide (EB) that can be used with either blue-light or UV excitation.

Safe DNA Gel Stain is a better nucleic acid staining reagent for all your molecular biology research needs. It is not only environmental friendly but also good for your sample.

Safe DNA Gel Stain is supplied as 10000X concentrate in DMSO and used in the same way as ethidium bromide solution. It is also suitable for staining RNA in gels.

Cloning efficiency of DNA fragments is improved by using Safe DNA Gel Stain and blue-light, compared with using ethidium bromide and UV light exposure.

1. Austin D C Miller, Soham P Chowdhury, et al. "Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types." bioRxiv. 2023 Nov 10:2023.11.07.566095. PMID: 37986852
2. Nicolas I E Rocos, Felicity J Coulter, et al. "The minor chicken class I gene BF1 is deleted between short imperfect direct repeats in the B14 and typical B15 major histocompatibility complex (MHC) haplotypes." Immunogenetics. 2023 Oct;75(5):455-464. PMID: 37405420
3. Zhichao Tang, Shalakha Hegde, et al. "Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5'untranslated region." bioRxiv. 2023 Oct 23:2023.04.03.535453. PMID: 37066172
4. Hannah Sleath, Bortolo Mognetti, et al. "Chemotactic crawling of multivalent vesicles along ligand-density gradients" Cornell University. 16 Oct 2023.
5. Wynne, Max Alexander, et al. "Investigation of the structure and function of SecH, a novel component of the Sec machinery in Escherichia coli" University of Birmingham. 2023.
6. Daniel Larcombe-Young, Lynsey Whilding, et al. "Generation of human parallel chimeric antigen receptor (pCAR) T cells to achieve synergistic T cell co-stimulation." STAR Protoc. 2022 May 21;3(2):101414. PMID: 35620078
7. Joseph Oddy, Rocío Alarcón-Reverte, et al. "Reduced free asparagine in wheat grain resulting from a natural deletion of TaASN-B2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality." BMC Plant Biol. 2021 Jun 29;21(1):302. PMID: 34187359
8. Soo Khim Chan, Zhongchao Zhao, et al. "Isolation of a Peptide That Binds toPseudomonas aeruginosaLytic Bacteriophage." ACS Omega 2022, 7, 42, 38053–38060.
9. Halle-Wittenberg. "SERF is a modifier of amyloid formation." Martin-Luther-Universität.
10. Shen Y, Ha W, et al. "Exome sequencing identifies novel mutation signatures of UV radiation and trichostatin A in primary human keratinocytes." Sci Rep. 2020;10(1):4943. PMID: 32188867
11. Urrutia G, Salmonson A, et al. "Combined Targeting of G9a and Checkpoint kinase 1 Synergistically Inhibits Pancreatic Cancer Cell Growth by Replication Fork Collapse." Mol Cancer Res. 2019 Dec 10. pii: molcanres.0490.2019. PMID: 31822519
12. Volkman HE, Cambier S, et al. "Tight nuclear tethering of cGAS is essential for preventing autoreactivity." Elife. 2019 Dec 6;8. pii: e47491. PMID: 31808743
13. Hannah E Volkman, Stephanie Cambier, et al. "cGAS is predominantly a nuclear protein." bioRxiv. 2018 December 04.

Hide ↑↑

• Reduce exposure to highly mutagenic EB and harmful UV light.
Safe DNA Gel Stain is less mutagenic and safer for you to work with than ethidium bromide. You can further decrease your exposure risk by using visible blue-light with Safe stain rather than UV. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.

• Increase sensitivity by reducing nonspecific background fluorescence.
Safe DNA Gel Stain offers excellent sensitivity in nucleic acid visualization with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. Plus, when used with blue light, Safe DNA Gel Stain has less background fluorescence than ethidium bromide–stained gels with UV light.

• Alternative to EB for all staining applications, including RNA staining.
Safe DNA Gel Stain is supplied as a concentrate in DMSO which can be used in the same way as ethidium bromide. It can be added into an agarose gel for staining during electrophoresis at a ratio of 1: 10000 or apply it after electrophoresis at a ratio of 1: 3300. Store at room temperature in its original packaging to avoid excessive light exposure.

Note: Safe DNA Gel Stain is less efficient in visualizing low molecule weight bands (100-200 bp).

Physical Appearance	DMSO solution
Storage	Store at 4°C, protect from light for 6 months.
M.Wt	504.66
Formula	C₂₈H₂₈N₂O₃S₂
Solubility	insoluble in EtOH; insoluble in H2O; ≥14.67 mg/mL in DMSO
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Purity = 99.91%

Safe DNA Gel Stain

Referência A8743-1ml

Contactar o distribuidor local :

Marca : APExBIO Technology

Contactar o distribuidor local :

Description

Product Citation

Features

Chemical Properties

Quality Control

Você também pode estar interessado nos seguintes produtos: