Sheep anti EBNA 3B

Background
EBNA-3B is one of only 10 viral proteins expressed in EBV-transformed B lymphoblasts cultured in vitro, through the expression of EBNA 3B is not required for EBV-mediated transformation in vitro. This 165kDa nuclear protein functions as a transcription factor.

Synonyms: EBNA3B; Epstein-Barr Virus nuclear antigen 4

Source
Sheep were immunized with recombinant EBNA-3B protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium sulfate precipitation

Concentration: See vial for concentration

Applications
Western blotting at 1-5 µg/ml

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Anderton, E., et al, 'Two Epstein-Barr virus (EBV) oncoproteins cooperate to repress expression of the proapoptotic tumour-suppressor Bim: clues to the pathogenesis of Burkitt's lymphoma' Oncogene 2007, 27, , 421-433 2. O'Nions, J., et al, 'Epstein–Barr virus can inhibit genotoxin-induced G1 arrest downstream of p53 by preventing the inactivation of CDK2' Oncogene 2003, 22, , 7181-7191 3. Jiménez-Ramírez, C., et al, 'Epstein-Barr Virus EBNA-3C Is Targeted to and Regulates Expression from the Bidirectional LMP-1/2B Promoter' Journal of Virology 2006, 80, , 11200-11208 4. Yuan, J., et al, 'Virus and Cell RNAs Expressed during Epstein-Barr Virus Replication' Journal of Virology 2006, 80, , 2548-2565 5. Hong, G.K., et al, 'Epstein-Barr Virus Lytic Infection Contributes to Lymphoproliferative Disease in a SCID Mouse Model' Journal of Virology 2005, 79, , 13993-14003 6. Rosendorff, A., et al, 'EBNA3C Coactivation with EBNA2 Requires a SUMO Homology Domain' Journal of Virology 2004, 78, , 367-377 7. Maruo, S., et al, 'Epstein-Barr Virus Nuclear Protein EBNA3A Is Critical for Maintaining Lymphoblastoid Cell Line Growth' Journal of Virology 2003, 77, , 10437-10447 8. Gordadze, A.V., et al, 'Notch1IC Partially Replaces EBNA2 Function in B Cells Immortalized by Epstein-Barr Virus' Journal of Virology 2001, 75, , 5899-5912

Protein Reference(s)

Database Name: UniProt

Accession Number: P03203 (EBV/HHV4)

Safety Datasheet(s) for this product:

NM_Sodium Azide