Vitamin E (VE) Colorimetric Assay Kit

Referência E-BC-K033-M-96T

Tamanho : 96T

Marca : Elabscience

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Detection Principle Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula.
Synonyms VE
Sample Type Serum,Plasma,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (525-533 nm, optimum wavelength: 533 nm)
Research Area Oxidative Stress ,  Plant Physiology Series
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Absolute ethanol, N-heptane
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.95 μg/mL
Detection Range 0.95-40 μg/mL
Precision inter-assay CV: 6.3% | intra-assay CV: 3.9%
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K034-M Vitamin C (VC) Colorimetric Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Mouse serum 1
Chicken serum 1
10% Mouse liver tissue homogenate 1
10% Mouse brain tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat lung tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat heart tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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