Mix qPCR for probes with high ROX

High ROX quantitative PCR mixes for probes are made for use with qPCR devices requiring high ROX as an internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations.

The mix for probes consists of all the necessary components (except template DNA, primers and probes): a Taq polymerase, dNTPs, MgCl2 and High ROX dye.

We offer a probe synthesis service for qPCR

Here's how to select the appropriate ROX dye for the qPCR device you are using:

Universal	All instruments
Without ROX	Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler.
Low ROX	Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P
High ROX	Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus